human neonatal dermal lymphatic endothelial cells (lec  (AngioBio Inc)

Journal: Cancer Science

Article Title: Suppression of lymph node and lung metastases of endometrial cancer by muscle‐mediated expression of soluble vascular endothelial growth factor receptor‐3

doi: 10.1111/cas.12184

Figure Lengend Snippet: Suppression of vascular endothelial growth factor (VEGF)‐C‐driven lymphatic endothelial cell (LEC) proliferation by conditioned medium of soluble vascular endothelial growth factor receptor‐3 (sVEGFR‐3)‐expressing cells. Cells were plated at 5 × 103 cells/well in 96‐well plates, and 50% sVEGFR‐3‐conditioned medium or luciferase‐conditioned medium was added with 100 ng/mL recombinant human VEGF‐C. The number of LEC with 50% sVEGFR‐3‐conditioned medium (b) was clearly smaller than that with control (a). The cells were counted by colorimetric assay 48 h after plating. Each bar represents the mean ± SD. (*P < 0.01) (c).

Article Snippet: Human neonatal dermal lymphatic endothelial cells (LEC) were purchased from AngioBio (Del Mar, CA, USA) and maintained in EGM‐MV2 BulletKit (Cambrex, East Rutherford, NJ, USA) supplemented with 10% inactivated FCS at 37°C in a 5% CO 2 atmosphere.

Techniques: Expressing, Luciferase, Recombinant, Colorimetric Assay

Journal:

Article Title: LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 Expression in Human Lymphatic Endothelium

doi: 10.1369/jhc.7A7299.2007

Figure Lengend Snippet: Expression of lymphatic endothelial markers. (A) Immunohistochemical analysis. Cells were visualized with rhodamine-conjugated Con A (Con A, in red), and lymphatic endothelial markers were visualized with an AF488-conjugated second antibody (AF488, in green). Merged images indicate that Prox1 and podoplanin are present in nuclei of human neonatal dermal lymphatic microvascular endothelial cells (LEC) and in the cytoplasm and cell membrane, but not in human umbilical vein endothelial cells (HUVEC). Bar = 100 μm. (B) RT-PCR analysis. The RT-PCR products of podoplanin mRNA were detected in LEC and increased with lipopolysaccharide (LPS). The RT-PCR products of Prox1 mRNA were detected in LEC but were not increased with LPS. The podoplanin and Prox1 mRNAs were not detected in HUVEC. MW, molecular weight marker. (C) Real-time quantitative PCR analysis. Podoplanin mRNA in LEC with 10 μg/ml LPS showed a 6.58-fold increase compared with LEC without LPS but was not detected in HUVEC. Prox1 mRNA did not increase in LEC with LPS and was rarely detected in HUVEC. Error bars indicate mean ± SEM. *Not significantly different from the untreated cells.

Article Snippet: Cell Culture Human neonatal dermal lymphatic microvascular endothelial cells (LEC) (HMVEC-dlyNeo; Cambrex Bio Science Walkersville, Inc., Walkersville, MD), and human umbilical vein endothelial cells (HUVEC) (CC-2505; Cambrex) were cultured in endothelial cell basal medium (EGM-2-MV Bulletkit; Cambrex).

Techniques: Expressing, Immunohistochemical staining, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker, Real-time Polymerase Chain Reaction

Journal:

Article Title: LPS-induced IL-6, IL-8, VCAM-1, and ICAM-1 Expression in Human Lymphatic Endothelium

doi: 10.1369/jhc.7A7299.2007

Figure Lengend Snippet: Expression of genes for lymphatic endothelial markers, and TLR-associated and leukocyte adhesion molecules in human neonatal dermal lymphatic microvascular endothelial cells with LPS treatments

Article Snippet: Cell Culture Human neonatal dermal lymphatic microvascular endothelial cells (LEC) (HMVEC-dlyNeo; Cambrex Bio Science Walkersville, Inc., Walkersville, MD), and human umbilical vein endothelial cells (HUVEC) (CC-2505; Cambrex) were cultured in endothelial cell basal medium (EGM-2-MV Bulletkit; Cambrex).

Techniques: Expressing, Marker, Activation Assay